Figure 3

Quantitative reverse transcript PCR analyses of Brachypodium distachyon IRIP genes at three timepoints after cold acclimation. (a) Bradi5g27300.1 (b) Bradi5g27310.1 (c) Bradi5g27330.1 (d) Bradi5g27350.1 The expression values are given as 2^-ΔΔCt and stars above bars denote the significance levels of t-test (*P < 0.05, **P < 0.001). Single extreme outliers were removed from four samples before estimation of expression levels and standard deviations (see Additional file 2).