Figure 4From: Chromosome arm-specific BAC end sequences permit comparative analysis of homoeologous chromosomes and genomes of polyploid wheatExample of the localization SSR and ISBP markers on chromosome 3AS and amplification across species. PCR products separated on 3 % agarose gels after amplification with one SSR (Xbem5) and one ISBP (Xisbp3A-14). Templates were DNA from (1) CS wheat, (2) a 3AS BAC library superpool, (3) N3AT3B, (4) Dt3AS, (5) Dt3AL, (6) T. monococcum subsp. monococcum TA4342-L96, (7) T. monococcum subsp. aegilopoides TA4342-L95, (8) T. turdigum subsp. durum cv. Langdon, (9) Langdon DIC-3A, (10) T. aestivum cv. Opata 85, (11) synthetic wheat W79849, (12) water, (L) 100-bp DNA ladder.Back to article page