Example of the localization SSR and ISBP markers on chromosome 3AS and amplification across species. PCR products separated on 3 % agarose gels after amplification with one SSR (Xbem5) and one ISBP (Xisbp3A-14). Templates were DNA from (1) CS wheat, (2) a 3AS BAC library superpool, (3) N3AT3B, (4) Dt3AS, (5) Dt3AL, (6) T. monococcum subsp. monococcum TA4342-L96, (7) T. monococcum subsp. aegilopoides TA4342-L95, (8) T. turdigum subsp. durum cv. Langdon, (9) Langdon DIC-3A, (10) T. aestivum cv. Opata 85, (11) synthetic wheat W79849, (12) water, (L) 100-bp DNA ladder.