Figure 3
Analysis of BA produced in corollas of ir-hd20 and WT plants. (A) The BA emitted from individual night flowers was trapped from 3 pm to 9 am and analyzed by GC-MS. The BA levels are expressed as the normalized peak areas to tetralin (internal standard; IS). Emitted BA was analyzed from both flowers that just opened their corollas (emitted first night) and from the same flowers but the day after (emitted second day). BA was also extracted from corolla tissue after the first night (retained in corollas after the first night). Asterisks denote significant differences; univariate ANOVA, F 2,37 = 5.65 P < 0.01 followed by a LSD post-hoc test P < 0.01 for both lines versus WT; n > 10; bars denote ± SE. (B) Kinetic of BA emission from flowers at different times during corolla opening. Emitted BA was analyzed in real time with a zNOSETM. Asteriks denote significant differences; univariate ANOVA, 10 pm: F 2,12 = 4.18 P < 0.05, 12 am: F 2,12 = 4.20 P < 0.05 followed by a LSD post-hoc test P < 0.05 for both lines versus WT; n > 10; bars denote ± SE).