Figure 5

Interaction of AtClpC2 and AtClpD with TPs located at the C-terminal end of GST. GST-TP or GST-TP1234 were loaded onto the glutathione-agarose resin. Blots show the remaining AtClpC2 (A) or AtClpD (B) after each washing step. Coomassie blue-stained SDS-PAGE gels below each blot show the integrity of GST fusion proteins along the procedure. Graphs indicate the percentage of residual AtClpC2 (C) or AtClpD (D) in the GST-TP (filled circles) or in the GST-TP1234-containing resin (filled diamonds) after each washing step. For AtClpD, only the lower band was plotted, as identical results were obtained with the upper band. Results from three different experiments are shown.