Figure 4

Binding of AtClpC2 and AtClpD to the wild-type TP located between GST and FNR. GST-FNR or GST-TP-FNR were used to load the gluthathione-agarose resin. Representative blots from three different experiments show the remaining AtClpC2 (A) or AtClpD (C) after each washing step. Graphs show the percentage of residual AtClpC2 (B) or AtClpD (D) in the GST-FNR- (hexagons) or GST-TP-FNR-containing resin (inverted triangles) after each washing step. For AtClpD, only the lower band was plotted, as identical results were obtained with the upper band. Coomassie blue-stained SDS-PAGE gels below each blot show the integrity of GST fusion proteins along the washing steps.