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Figure 3 | BMC Plant Biology

Figure 3

From: Chloroplastic Hsp100 chaperones ClpC2 and ClpD interact in vitro with a transit peptide only when it is located at the N-terminus of a protein

Figure 3

Limited proteolysis assays. GST-TP-FNR was digested with thermolysin. At different time points (see Materials and Methods), aliquots were taken and analyzed by SDS-PAGE stained with Coomassie Brilliant Blue (A), followed by immublotting revealed with antibodies raised against GST (B) or FNR (C). Lanes 1 show the migration of molecular weight standards.

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