Depiction of the fusion proteins used in this study. (A) Amino acid sequence of wild type TP and TP1234. Substituted amino acids are shown in bold. (B) Proteins used throughout the study. Yellow boxes represent either TP (5 kDa), black boxes GST (27 kDa) and red boxes FNR (35 kDa). (C) Purity and integrity of GST and GST-fusions, checked by SDS-PAGE followed by Coomassie blue staining. Left lane: molecular weight standard.