Dual ER/tonoplast localization of DMP1-eGFP in stably transformed A. thaliana plants. Overexpression of DMP1-eGFP in in young emerging Arabidopsis cotyledons (7 DAS) leads to labeling of the tonoplast and bulbs (a), the ER network (a, arrow and inset) and ER bodies (a, inset). In 12 DAS cotyledons the number of bulbs decreases (b) and at 18 DAS bulbs are no longer visible (c). In rosette leaves intense ER labeling is observed (d). In hypocotyls the ER is associated with single ER bodies (c), with ER body clusters (f), or with large ER body aggregates extending across the whole cell (g). Massive vesiculation of endomembranes occurs during dark induced senescence (h). Scale bar, 10 μm.