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Table 2 HPLC analysis of leaf carotenoid content (μg/g FW) in dark-adapted plants

From: A quadruple mutant of Arabidopsis reveals a β-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio

  Carotenoid content (μg/g FW)
  neoxanthin violaxanthin antheraxanthin lutein zeaxanthin α-carotene β-carotene β-β-xantophylls xanthophylls/carotenes
WT 18.6 ± 1.6a 44.1 ± 5.8a nd 105.6 ± 22.4a nd 4.4 ± 0.6a 62.1 ± 14.8a 62.7 ± 6.0a 2.5 ± 0.7a
lut2 22.1 ± 0.7b 94.7 ± 4.9b 14.6 ± 1.4a nd 5.0 ± 0.6a nd 74.9 ± 5.1a 136.4 ± 5.1b 1.8 ± 0.1a
chy1chy2lut5 0.3 ± 0.2c 0.9 ± 0.6c nd 69.1 ± 4.7b nd 51.0 ± 2.7b 42.8 ± 1.4b 1.2 ± 0.6c 0.7 ± 0.1b
chy1chy2lut2lut5 8.4 ± 0.1d 13.6 ± 0.2d 8.5 ± 0.1b nd 3.4 ± 0.1b nd 109.4 ± 2.3c 33.9 ± 0.3d 0.3 ± 0.1c
  1. Plants grown at 30 μmol photons m-2 s-1 for 6 weeks were dark-adapted, then carotenoids were extracted and quantified via LC-DAD-MS. Data are expressed as mean ± SD (n = 4). nd, not detectable; FW, fresh weight. Values marked with the same letters are not significantly different from each other within a column (P > 0.05).