Figure 4

Cytosine methylation changes in a fragment of Osr36 (portion of the 5'-LTR-region) in the S0 generation of the etoposide-treated plants of Nipponbare, determined by bisulfite sequencing. Ten clones were arbitrarily sequenced for the control and the 10 mg/L etoposide-treated plants, respectively. All three types of cytosines, CG (red circles), CHG (blue circles) and CHH (green circles) were shown in the map. Filled and empty circles denote methylated and unmethylated cytosines, respectively. The red, blue and green columns in the histograms refer to the collective methylation levels (in percentage) respectively of CG, CHG and CHH, of the LTR-region. The seven CHG sites that showed dramatic hypermethylation were arrowed. The nucleotide sequence of this analyzed fragment was presented in Additional file 1.