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Figure 2 | BMC Plant Biology

Figure 2

From: Changes in DNA methylation and transgenerational mobilization of a transposable element (mPing) by the Topoisomerase II inhibitor, Etoposide, in rice

Figure 2

Changes in DNA methylation in the S0 generation of etoposide- treated plants of genotypes Matsumae, RZ1, RZ2 and RZ35, relative to their respective control plants. Each genotype was treated by two concentrations of etoposide, 10 mg/L and 20 mg/L, respectively. “Control” represents plants treated with ddH2O. Notably, DNA methylation changes occurred in all three recombinant inbred lines (RZ1, RZ2 and RZ35) but not in their rice parental line Matsumae. Also, the changes occurred in either or both of the enzyme digests, though more abundantly in MspI-digest, and the changes occurred either in the 10 mg/L concentration or in both concentrations. The OsCDPK protein-encoding gene is intrinsically unmethylated and thus showed a monomorphic banding pattern among all treated plants in both HpaII and MspI digestions across the genotypes, indicating complete digestion in all samples.

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