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Table 3 Primer sequences for quantitative real-time PCR

From: Generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation

Gene name Primer sequences-forward (F) and reverse (R) Target Tissue
1-aminocyclopropane-1-carboxylate oxidase F-TGCAGGTGCCGGGTAGAT
R-ATGTCCCTAGCCTCGTTCTACAAC
Fruit
Abscisic stress ripening F-CCAATTTCACAACCTCGCTCTA
R-TGGTGATCGGAAAAGGAAAAA
Bud
Amino acid selective channel protein F-AATGGAGTATGGGATGGAGAGAGT
R-CCCCGAGCATTGCATTCTT
Bud
Anthocyanidin synthase F-TTCGTGGGCGGCTTTCT
R-GAGCGGCTTCAGGATGATCT
Fruit
Arginine decarboxylase F-CGCTTCGTGCGAGCTAGATA
R-TGCAATAGTCTCTCACCACTCGAT
Bud
Aspartic proteinase F-GAGTGCCATCGGTATAGCACAA
R-CACAGGCAGAGCCCCAAA
Fruit
Chalcone synthase F-AGGCCTTCCAGCCTTTGG
R-GGTGGGCGATCCAGAAGAT
Fruit
Cysteine protease-like protein F-CAACGGTTGAGACCATGGAAT
R-TGCAGAGGTCCCCATGCT
Fruit
Dehydrin-14 kDa F-CGCGGCGATTAGATCGAA
R-AGCTAGCGTAGAGGCGGAAA
Bud
Flavonoid 3-hydroxylase F-GCCGGTGCAACCCACTT
R-GGCCGTAGTTGGCGAAAAC
Fruit
Galactinol synthase F-AAGGGTGTGGTGGGATTGG
R-CCAAAGGGTACGCGC CTT
Bud
Glutathione peroxidase F-TCTAGCATCCTTGACGGTGAAG
R-GGCAAGCCAATCCGAGAAG
Fruit
High mobility group family protein F-CGTCATGAAAGGAGGTAAATCCA
R-TTTCGCAGTTTTCTTCACCGATA
Bud/Fruit housekeeping gene
Hypothetical protein F-CCTGCTGCATCAAGCTGTTG
R-TGGCTGGAAGCACTCACTGT
Bud/Fruit housekeeping gene
Lipid transfer protein F-CCAAGGTGAAATGAGCAAGGA
R-GCTTCATCCCGCGACATG
Bud
Lipoxygenase F-CCAGCTGGATGTGGCAACTT
R-ACGCCTGCGTCATTGGA
Bud
Metallothionein F-ACCCTGACATGAGCTTCTCG
R-ACCCAAATCTCTGCTTGCTG
Bud housekeeping gene
Pectate lyase F-GGTTGCCGGTCCCACAA
R-GAGCATCAATGCGTCAAGGA
Fruit
Pointed first leaf F-CGTTTTGCCAACATCGTTTG
R-GCTCACCAGCCCTCTTGTTC
Bud
Pyrophosphate-dependent phosphofructokinase F-GCCCTTAACAACCGCTACATCA
R-TCTGCTACTGGGCCAACAAAT
Bud
S-adenosyl-1-homocysteine hydrolase F-CACCGGATCGCTTCACATG
R-CGGTGAGGGTTTCGATAAGG
Fruit housekeeping gene