Table 3 Primer sequences for quantitative real-time PCR
Gene name | Primer sequences-forward (F) and reverse (R) | Target Tissue |
|---|---|---|
1-aminocyclopropane-1-carboxylate oxidase | F-TGCAGGTGCCGGGTAGAT R-ATGTCCCTAGCCTCGTTCTACAAC | Fruit |
Abscisic stress ripening | F-CCAATTTCACAACCTCGCTCTA R-TGGTGATCGGAAAAGGAAAAA | Bud |
Amino acid selective channel protein | F-AATGGAGTATGGGATGGAGAGAGT R-CCCCGAGCATTGCATTCTT | Bud |
Anthocyanidin synthase | F-TTCGTGGGCGGCTTTCT R-GAGCGGCTTCAGGATGATCT | Fruit |
Arginine decarboxylase | F-CGCTTCGTGCGAGCTAGATA R-TGCAATAGTCTCTCACCACTCGAT | Bud |
Aspartic proteinase | F-GAGTGCCATCGGTATAGCACAA R-CACAGGCAGAGCCCCAAA | Fruit |
Chalcone synthase | F-AGGCCTTCCAGCCTTTGG R-GGTGGGCGATCCAGAAGAT | Fruit |
Cysteine protease-like protein | F-CAACGGTTGAGACCATGGAAT R-TGCAGAGGTCCCCATGCT | Fruit |
Dehydrin-14 kDa | F-CGCGGCGATTAGATCGAA R-AGCTAGCGTAGAGGCGGAAA | Bud |
Flavonoid 3-hydroxylase | F-GCCGGTGCAACCCACTT R-GGCCGTAGTTGGCGAAAAC | Fruit |
Galactinol synthase | F-AAGGGTGTGGTGGGATTGG R-CCAAAGGGTACGCGC CTT | Bud |
Glutathione peroxidase | F-TCTAGCATCCTTGACGGTGAAG R-GGCAAGCCAATCCGAGAAG | Fruit |
High mobility group family protein | F-CGTCATGAAAGGAGGTAAATCCA R-TTTCGCAGTTTTCTTCACCGATA | Bud/Fruit housekeeping gene |
Hypothetical protein | F-CCTGCTGCATCAAGCTGTTG R-TGGCTGGAAGCACTCACTGT | Bud/Fruit housekeeping gene |
Lipid transfer protein | F-CCAAGGTGAAATGAGCAAGGA R-GCTTCATCCCGCGACATG | Bud |
Lipoxygenase | F-CCAGCTGGATGTGGCAACTT R-ACGCCTGCGTCATTGGA | Bud |
Metallothionein | F-ACCCTGACATGAGCTTCTCG R-ACCCAAATCTCTGCTTGCTG | Bud housekeeping gene |
Pectate lyase | F-GGTTGCCGGTCCCACAA R-GAGCATCAATGCGTCAAGGA | Fruit |
Pointed first leaf | F-CGTTTTGCCAACATCGTTTG R-GCTCACCAGCCCTCTTGTTC | Bud |
Pyrophosphate-dependent phosphofructokinase | F-GCCCTTAACAACCGCTACATCA R-TCTGCTACTGGGCCAACAAAT | Bud |
S-adenosyl-1-homocysteine hydrolase | F-CACCGGATCGCTTCACATG R-CGGTGAGGGTTTCGATAAGG | Fruit housekeeping gene |