Table 3 Primer sequences and restriction enzymes for the RGL-tagged CAPS markers

PvRGL016

TGCACCATGACTGTTCTCCACC

AATACCACCTCCAGCCCAAGA

HindIII/G

PvRGL039

CTACTCCCTCAAAGGAATCACC

TGAGAAAGTTTTTGCTCAATGG

DraI/B, A

PvRGL040

TCTTAAAGGACGCACCTACACG

ATTGGCCTTCAAGACGGTTAT

SnaBI/G

PvRGL041

GCCTCCGTTTGAAAGTTGCTC

CACGAAATATCTTACGTTTGTTTGC

AgeI/G

PvRGL049

GAACAATGTTAGTAGTACTTCGCC

GGCTTCCTCTTGAGAGTACTTTG

PflMI/S, O, G, B, P

PvRGL050

CACATTAGTAGCCTTTATGTCCC

CTTCGGTCCTGTCTACAAGGT

HindIII/G

PvRGL065

CGCTTAGGAGTTGGTAGAGTAACAC

TCAAGGAAACACCGACAGAATG

NsiI/S, O, B, P, A

PvRGL073

CCAAAATCAGAAACTTTGGCATG

AGAGCCCTGGGCAGGAAAGAC

AflII/G

PvRGL093

TGGGTTACTGTTGTGACGATGT

TATGTGCCCATGATCCTGGTAG

DraI/O, P

PvRGL099

CTTCGCCATCTCCTTGTCTATC

GCCCTATGCTCAGCCTTTCTAG

BglII/B, G

PvRGL102

TGAAGCCCTTATGCACAGGTCC

TCATCATCGCCAAACCCCAAC

XmnI/S, O, P

PvRGL153

AGTGATTTCCATTTATACGAGG

AAAAGCCAAGTAACATTCTTCT

SphI/O

PvRGL155

GAACCAGCAATTTGTTACCGAG

TAGTTGTTGAGCAACCTTTAGC

NdeI/O, B, P

PvRGL173

GGATCATTGCCTGTATTCGAGG

TGGCACTGTCCCTGATAAACTG

EcoRI/G, B, A

PvRGL246

CAGCCACCTTGGGTTGGTAATC

GACGCAGTTGCTAAATGGACACC

HindIII/S, O, P

PvRGL264

GTAACTCGTGTCCTCCTCATCT

GATACTTGAGAAGGTAAAGGCTG

SpeI/B

PvRGL297

TTGGAGCAATGAGAAAATAGGGG

TCCAGCAGAAGCCCTGTGATG

SpeI/G

PvRGL302

CTCTTAAAGGACGCACCTACAC

TATTGGCCTTCAAGACGGTTAT

SnaBI/G

PvRGL308

TAAGATGTGTCAATAAATATGCTG

CAGGAAGTTGCTGTAAAGAAGT

BspMI/G