Figure 3

Phyogenetic footprinting of BB promoter. a) VISTA plot of pair-wise comparisons of different Brassicaceae and A. thaliana. Graph illustrates a 20 bp sliding window comparison and conservation of more than 90% with a minimum width of 20 bp is marked colour (dark blue for exon in 5' UTR, light blue for intron in 5' UTR, red for non-transcribed sequence). Blocks of high conservation are indicated as boxes, black elements were analysed further and the red box was not analyzed as no binding motif was predicted by FootPrinter. b) Sequence alignement of the non-transcribed BB promoter. Highly conserved blocks are underlined. Motifs predicted by FootPrinter are indicated as red boxes. ARF binding sites are shown as blue boxes and MYB binding site as orange box. The blue arrow above positions 370 to 400 indicates 5' UTR sequence. c) bb-1 rescue experiment using different Brassicaceae promoters normalised with L.er. Error bars indicate SE and significant changes to L.er are marked with * (p < 0.05). All measurements are significantly different to bb-1 (p < 0.03). d) Auxin induction assay measuring LUC expression after 1h (T1) and 3h (T2). All measurements were normalized to the values for the H₂O control at the respective time points, error bars are SE.