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Figure 5 | BMC Plant Biology

Figure 5

From: Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2

Figure 5

( A ) DGAT1 expression Lane 1: WT; Lane 2 lpcat2 H single mutant, Lane 3- AS11 dgat1 single mutant; Lane 4 dgat1/lpcat2 H/H double mutant line #6-3-10-19; Lane 5 dgat1/lpcat2 H/H double mutant line #6-3-20-1. (B) LPCAT2 expression: Lane 1: WT; Lane 2 lpcat2 H single mutant SAIL_357_H01, Lane 3-AS11 dgat1 single mutant; Lane 4 dgat1/lpcat2 H/H double mutant line #6-3-10-19; Lane 5 dgat1/lpcat2 H/H double mutant line #6-3-20-1. RT-PCR was conducted using DGAT1-specific primers (A) and LPCAT2-specific primers (B) as described in "Methods". The maps show the position of 147 bp repeat insertion in the AS11 DGAT1 gene and the T-DNA insertion site in the LPCAT2 gene.

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BMC Plant Biology

ISSN: 1471-2229

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