Figure 2
Expression of the mutated A7 and AS11 DGAT1 and WT DGAT1 cDNAs in H1246MATα yeast cells lacking DGAT1 activity. The insert photo (A) shows the western blot of the DGAT protein expressed in each lysate with the 52 kD and 75 kD Mr marker positions indicated by the arrows. (B) DGAT activity in each lysate (measured as pmol 14 C triolein formed · min-1 · (mg protein)-1 in the presence of 14 C-1,2 diolein and 18:1-CoA) is reported as the average of triplicate determinations ± S.D. Only the expressed WT protein shows DGAT activity, confirming that both the AS11 and A7 DGAT1s are non-functional.