Figure 3

HY1 and HY5 a dditively regulate the light-induced gene expression. A - B, Relative expression of CAB1and RBCS-1A in 6-day-old seedlings grown in WL (90 μmol m^-2 s^-1). C, Accumulation of chlorophyll in 6-day-old wild-type and mutant seedlings grown in WL (90 μmol m^-2 s^-1). D, Accumulation of anthocyanin in 6-day-old wild-type and mutant seedlings grown in WL (90 μmol m^-2 s^-1). The error bars indicate SD. *** - indicates significant difference from hy5 (p > 0.001 student's t-test, n = 30, number of seedlings used for hypocotyl measurement). Real-time PCR was repeated more than thrice and in each biological experiment three technical replicates were used. Similar results were obtained in all the experiments. A representative figure is shown here. For chlorophyll and anthocyanin estimation, 50 seedlings was used in each genotype and the experiment was repeated thrice and in each biological experiment, four technical replicated were used. Similar results were obtained in all the experiments. A representative figure is presented.