Figure 7
From: Proteomic characterisation of endoplasmic reticulum-derived protein bodies in tobacco leaves
Main features of PB's structure inferred from proteome analysis. a) Both the membrane and most chloroplastic proteins as well as proteins involved in protein synthesis and transporters are directly accessible help to SDS (white regions). Most proteins involved in protein folding or degradation and in response to stress (light grey) become accessible after disruption of disulfide bonds, but part of them requires additional heating due to likely entrapment into Zera-DsRed polymers (dark grey) that resists to reductant (100 mM DTT) at room temperature. b) Immunoelectron microscopy of PBs showing some heterogeneity in the internal structure.