Figure 6
From: Proteomic characterisation of endoplasmic reticulum-derived protein bodies in tobacco leaves
Recruitment of ER-soluble proteins in Zera-DsRed PBs. Confocal microscopy images of N. benthamiana epidermal cells a) transformed only with SP-ECFP-KDEL construct, scale bar 20 ÎĽm. b-d) co-transformed with Zera-DsRed and SP-ECFP-KDEL constructs. (b) ER labelled (cyan) by the expression of ECFP-KDEL, a luminal ER protein. (c) Fluorescent spots (red) corresponding to Zera-DsRed-PBs and (d) Image shows the merge of both fluorescent labels illustrating the presence of luminal-ER proteins in PBs. Scale bar: 2 ÎĽm.