Figure 5

HPLC analysis of pigments produced in antirrhinum petals transiently transformed with 35S: PgDOD or 35S: GFP. HPLC analysis was conducted on the 35S: PgDOD and 35S: GFP antirrhinum petal material shown in Figure 3. Absorbance was monitored at 470 nm for betaxanthins or 538 nm for betacyanins. Chromatograms are for extracts from beetroot root (A and E), 35S: PgDOD antirrhinum infiltrated with L-DOPA (B and F), 35S: PgDOD antirrhinum without L-DOPA infiltration (C and G), 35S: GFP antirrhinum infiltrated with L-DOPA (D and H). The retention times of the major peaks are shown, and these were tentatively identified as per Table 1.