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Table 2 Names and composition of dialysis buffers

From: Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germination

Names of dialysis buffers Composition
Buffer 6 M 50 mM potassium phosphate pH10.7, 5 mM EDTA, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, 6 M urea
Buffer 4 M 50 mM potassium phosphate pH10.7, 5 mM EDTA, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, 4 M urea
Buffer 2 M 50 mM potassium phosphate pH10.7, 5 mM EDTA, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, 2 M urea
Buffer 0 M 50 mM potassium phosphate pH10.7, 5 mM EDTA, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, 0 M urea