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Table 1 Primer and TAQMAN probes used for the quantitative PCR experiments.

From: Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germination

Primers and Probes

Sequences

rpl39-F1

5'GAACAGGCCCATCCCTTATTG3'

rpl39-R1

5'CGGCGCTTGGCATTGTA3'

rpl39-MGB1

5' ATGCGCACTGACAACA 3'

CP1-F1

5'ACACAGACCTCTTGATACCAAAACAT3'

CP1-R1

5'TCTTCCAAGAGCAAACCACCTT3'

CP1-MGB1

5' TCTGCTCTTCAGAGGTTGTA 3'

CP4-F1

5'CAGGATGCAACGTGGTGTTG3'

CP4-R1

5'CCTCCATTGCTATCCCACAAA3'

CP4-MGB1

5' TGCTGCTGAAGGCG 3'

CPI1-F1

5'TGTTTGGGAGATCTAATCTGATGATT3'

CPI1-R1

5'AAACCGAACACTTAACAGCAAAGA3'

CPI1-MGB1

5' TTAGTACCTTTCAGTGCAAAT 3'

CPI2-F1

5'CGCTATTGCCTATTTGCCTAGTAGA3'

CPI2-R1

5'GAAACTCCAATCTTTCCAACTGAAA3'

CPI2-MGB1

5' TAAAGCTAACGCGTAAATG 3'

CPI3-F1

5'AACCGACGCTGCAAGAATG3'

CPI3-R1

5'CAGGGTGGTGAGTAGGAGGAGAT3'

CPI3-MGB1

5' CTTCTGCCTTTCCC 3'

CPI4-F1

5'TGTTTATGGTGTGGCTTTCAGTTT3'

CPI4-R1

5'CGTAGGGAGACGTATGCATGAC3'

CPI4-MGB1

5' TGCATGGATGATGTACTG 3'

  1. All MGB Probes were labeled at the 5' end with the fluorescent reporter dye 6-carboxyfluorescein (FAM) and at the 3' end with the quencher dye 6-carboxy-tetramethyl-rhodamine (TAMRA), except rpl39 probe which was labeled at the 5' end with the fluorescent reporter dye VIC and at the 3' end with quencher TAMRA