Figure 4From: Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germinationDetection of CcCP4 protease activity. The assay was run for different lengths of time as described in the Materials and Methods and samples were then run on SDS-PAGE gels followed by coomassie staininig. The markers (lane M) as in Figure 3. 'Activated' indicates HIS-SUMO-CP4 which has been acid treated. T = reaction times with BSA.Back to article page