Figure 2

Quantitative expression analysis of CcCP1 and CcCP4 in different tissues and at different stages for Robusta BP409. The expression of each gene was measured in the various maturing and germinating grain samples, as well as maturing pericarp samples plus in roots, branches, and leaves. For germination stages T5 and T6, only the grain (T5) or grain/emerging cotyledons were extracted for RNA. The expression levels were obtained using quantitative RT-PCR. RQ is the expression level of each gene relative to the constitutively expressed gene RPL39. GSG, small green stage grain; GLG, large green stage grain; GY, yellow stage grain; GR, red stage grain. PSG, pericarp small green stage; PLG, pericarp large green stage; PY, pericarp yellow stage; PR, pericarp red stage. T1 is the sterilized and washed material obtained just before placing on the "germination" media; T2 = 3 DAI, T3 = 5 DAI, T4 = 14 DAI (FE), T5 = FE + 1 month, T6 = FE + 2 months. DAI represents "Days After Imbibition", FE represents "First Evidence" of germination.