Table 2 Novel tobacco miRNAs identified in this study
From: Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum)
Novel miRNAs | Size (nt) | Pre-miRNA hit | Number of the mature miRNA reads (RPM) | Mature miRNA sequence | |||
|---|---|---|---|---|---|---|---|
| Â | Â | Â | Root | Leaf | Â | ||
| Â | Â | Â | Control | Topping | Wounding | Wounding | Â |
Nta-miR1 | 21 | ET738113.1 | 2.3 | 16.6 | 204.9 | 123.7 | TGGCAACTTCTTCATCATGCC |
Nta-miR2 | 21 | FH134894.1 | 12.7 | 24.5 | 28.1 | 15.2 | TCAATTGAGATGACATCTAGT |
Nta-miR3 | 22 | FH179115.1 | 12.7 | 20.6 | 2.4 | 1.9 | CATTTTCACATGTAGCACTGAC |
Nta-miR4a.1 | 22 | ET742627.1 | 16.1 | 14.9 | 11.6 | 3.7 | AACAATTGAGATAACATCTAGG |
Nta-miR4b | 22 | FH277271.1 | 16.1 | 14.9 | 11.6 | 3.7 | TGCCAACTATTGAGATGACATC |
Nta-miR4a.2 | 22 | ET742627.1 | 5.2 | 14.5 | 719.5 | 396.2 | TGCCAACTATTGAGATGACATC |
Nta-miR4c | 22 | ET817133.1 | 0.3 | 0.2 | 9.4 | 6.6 | TGCCAATTATAGAGATGACATC |
Nta-miR5a | 22 | FH599068.1 | 4.9 | 4.2 | 2.5 | 1.5 | TTTGTCCAATGAAACACTTATC |
Nta-miR5b | 22 | ET748842.1 | 70.9 | 45.9 | 21.0 | 25.6 | TTTGTCCAATGAAATACTTATC |
Nta-miR6 | 21 | FH715144.1 | 1.6 | 3.5 | 4.7 | 5.3 | TGACATCTTCAAAACCCACTA |
Nta-miR7 | 21 | ET849487.1 | 6.8 | 8.7 | 0.2 | 0.0 | TACGTCGATCGATTGTTCTTA |
Nta-miR8 | 21 | ET878171.1 | 5.9 | 1.9 | 3.3 | 0.2 | TGTGTTAATCGTTTGTTCTCA |
Nta-miR9a | 21 | ET872202.1 | 14911.6 | 29389.3 | 2155.8 | 1094.8 | TTGATACGCACCTGAATCGGC |
Nta-miR9b | 21 | ET886878.1 | 14911.6 | 29389.3 | 2155.8 | 1094.8 | TTGATACGCACCTGAATCGGC |
Nta-miR10 | 22 | FH007932.1 | 4.2 | 7.7 | 42.8 | 21.6 | TACAGGTGACTTGTAAATGTTT |
Nta-miR11 | 22 | FH363222.1 | 2.9 | 3.9 | 1.3 | 1.5 | AGATTTGTTTGATCGTCTTGGC |
Nta-miR12 | 22 | FH007932.1 | 4.6 | 1.9 | 224.3 | 171.1 | AGATACTCAGCAAAACATTTAC |
Nta-miR13a | 22 | ET680982.1 | 0.2 | 0.4 | 1.3 | 0.6 | TGAATGTGAGGCATTGGATTGA |
Nta-miR13b | 22 | ET781387.1 | 0.2 | 0.4 | 1.3 | 0.6 | TGAATGTGAGGCATTGGATTGA |
Nta-miR13c | 22 | ET788684.1 | 0.2 | 0.4 | 1.3 | 0.6 | TGAATGTGAGGCATTGGATTGA |
Nta-miR13d | 22 | ET967981.1 | 0.2 | 0.4 | 1.3 | 0.6 | TGAATGTGAGGCATTGGATTGA |
Nta-miR13e | 22 | FH063511.1 | 0.2 | 0.4 | 1.3 | 0.6 | TGAATGTGAGGCATTGGATTGA |
Nta-miR13f | 22 | ET676102.1 | 0.2 | 0.4 | 0.4 | 0.2 | TGAGTGTGAGGCATTGGATTGA |
Nta-miR13g | 22 | ET780880.1 | 4.9 | 9.4 | 1.5 | 1.6 | TGAGTGTGAGGCGTTGGATTGA |
Nta-miR13h | 22 | ET858637.1 | 4.9 | 9.4 | 1.5 | 1.6 | TGAGTGTGAGGCGTTGGATTGA |
Nta-miR13i | 22 | FH085512.1 | 4.9 | 9.4 | 1.5 | 1.6 | TGAGTGTGAGGCGTTGGATTGA |
Nta-miR14 | 21 | ET964891.1 | 9.4 | 65.0 | 17.6 | 10.2 | TGAGTGTGAGGCGTTGGATTGA |
Nta-miR15a | 24 | ET740537.1 | 0.0 | 0.0 | 3.4 | 3.2 | TATTGTATTCGACTGTATTCACGG |
Nta-miR15b | 24 | FH095578.1 | 0.0 | 0.0 | 3.4 | 3.2 | TATTGTATTCGACTGTATTCACGG |
Nta-miR16 | 21 | FH440616.1 | 2.3 | 0.8 | 5.6 | 1.9 | TTATCATACGTAGCACTAGCC |
Nta-miR17 | 21 | FH731935.1 | 354.2 | 52.6 | 116.5 | 54.4 | TAGGACCATATTCACTATTTG |
Nta-miR18a | 21 | ET783551.1 | 3.3 | 1.2 | 24.2 | 23.1 | TGGGTCTCCTGGAGAAAGGTC |
Nta-miR18b | 21 | FH988094.1 | 3.3 | 1.2 | 24.2 | 23.1 | TGGGTCTCCTGGAGAAAGGTC |
Nta-miR19 | 21 | ET839854.1 | 2.3 | 6.0 | 13.4 | 5.1 | TAAGGTTGCCTTGCTCTTGCA |
Nta-miR20 | 21 | FH374163.1 | 2.9 | 1.2 | 23.8 | 11.9 | AGTGGGTGGAGTGGTAAGATA |
Nta-miR21 | 20 | FH383824.1 | 0.0 | 0.2 | 10.0 | 1.7 | CAGTGCACATATAACAGTAA |
Nta-miR22 | 21 | ET704987.1 | 0.0 | 0.2 | 8.0 | 1.1 | TTGAAGATGTTCTATTTCTGT |
Nta-miR23 | 22 | ET679542.1 | 58.5 | 62.3 | 0.4 | 0.2 | TGGTAGACGTAGGATTTGAAGA |
Nta-miR24a | 21 | FH052337.1 | 0.2 | 1.3 | 0.1 | 0.1 | AAAATGTGGCCGGATACGTGT |
Nta-miR24b | 21 | FH324277.1 | 0.2 | 1.3 | 0.1 | 0.1 | AAAATGTGGCCGGATACGTGT |
Nta-miR25 | 22 | FH081190.1 | 2.6 | 20.2 | 389.8 | 12.9 | TGAACTCTCTCCCTCAATGGCT |
Nta-miR26a | 21 | ET837426.1 | 35.1 | 179.9 | 2.2 | 0.4 | ATTGTTACATGTAACACTGGC |
Nta-miR26b | 21 | FH393496.1 | 483.9 | 1146.6 | 1.5 | 1.3 | ATTGTTACATGTAGCACTGGC |
Nta-miR27a | 22 | FH905151.1 | 0.7 | 1.3 | 0.2 | 0.1 | AAGTTCGATTTGTACGAAGGGC |
Nta-miR27b | 22 | FH915892.1 | 0.7 | 1.3 | 0.2 | 0.1 | AAGTTCGATTTGTACGAAGGGC |
Nta-miR27c | 22 | FH918314.1 | 0.7 | 1.3 | 0.2 | 0.1 | AAGTTCGATTTGTACGAAGGGC |
Nta-miR28 | 22 | FH253762.1 | 11.4 | 11.4 | 13.4 | 12.3 | TAGCATAGAATTCTCGCACCTA |
Nta-miR29 | 21 | FH324206.1 | 0.0 | 2.1 | 0.0 | 0.0 | ACGGGTGCGGCTACATTTTGG |
Nta-miR30 | 21 | FI086539.1 | 16.6 | 7.7 | 2.2 | 0.2 | ATCGTAACATATAGCACTAGC |
Nta-miR31 | 24 | ET823610.1 | 0.0 | 0.0 | 0.9 | 0.6 | GCATATATGGGCCAACTGTGTAAC |
Nta-miR32 | 21 | FH560937.1 | 0.0 | 0.0 | 1.6 | 1.7 | TGAACTCCAGCATATTATACT |
Nta-miR33a | 24 | FH761979.1 | 0.2 | 0.4 | 4.0 | 5.3 | GCTGGACCGGTATACTTTGCTGAC |
Nta-miR33b | 24 | FH762383.1 | 0.2 | 0.4 | 4.0 | 5.3 | GCTGGACCGGTATACTTTGCTGAC |
Nta-miR34 | 22 | ET840416.1 | 1.6 | 24.7 | 13.2 | 7.8 | TTCCCGACTCCCCCCATACCAC |
Nta-miR35 | 21 | FH119649.1 | 0.0 | 0.0 | 5.4 | 1.1 | AGAAAAATGGTAGCCATTGGA |
Nta-miR36 | 24 | FH408012.1 | 0.0 | 0.0 | 21.6 | 27.3 | AATATACTGGAGTTCGGTGCACCT |
Nta-miR37 | 22 | FH666318.1 | 0.0 | 0.0 | 3.3 | 0.4 | TGGAAGTACTGCCTAAGTTTGA |
Nta-miR38a | 21 | FH681839.1 | 0.8 | 1.0 | 0.5 | 0.1 | TCACATAAATTGAAACGGAGG |
Nta-miR38b | 21 | FH968988.1 | 0.8 | 1.0 | 0.5 | 0.1 | TCACATAAATTGAAACGGAGG |