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Table 2 Activation of promoters Pchs_H1 and Pchs4 with selected Myb and hop TFs in various combinations

From: Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus LupulusL.)

Myb (M) AC M MB* MW** MB*W**
     Pchs_H1  
HlMyb2 FN646081 (+) + (+) ++
s-HlMyb3 AM501509 0 + 0 ++
l-HlMyb3 AM501509 0 + 0 0
HlMyb1# AJ876882 0 (+) n.d. (++)
HlMyb7 AB292244 0 0 0 0
AtMyb23 NM_123397 (+) (+) + ++
AtMyb12 NM_130314 ++ ++ ++ 0
AtMyb75# NM_104541 + + + ++
     Pchs4  
HlMyb2 FN646081 0 0 0 (+)
s-HlMyb3 AM501509 + + + 0
l-HlMyb3 AM501509 + (++) 0 0
HlMyb7 AB292244 0 0 0 0
AtMyb23 NM_123397 0 0 (+) 0
AtMyb12 NM_130314 + 0 + 0
AtMyb75$ NM_104541 0 (+) 0 (+)
  1. * B = HlbHLH2 (AC: FR751553)
  2. **W = HlWD40_1 (AC: FN989721)
  3. $ AtPAP1;
  4. #not tested for Pchs4
  5. n.d. not determined
  6. (+) up to 10% of maximal level
  7. + up to 70% of maximal level
  8. ++ maximum activity observed; (++) low maximal activity; the maximal levels for M/promoter/combination/pmol GUS/mg/min: HlMyb2/PchsH1/MBW/5127; s-HlMyb3/PchsH1/MBW/1192; l-HlMyb3/Pchs4/MB/511; HlMyb1/Pchs_H1/MBW/304; AtMyb23/P_chsH1/MBW/2036; AtMyb12/P_chsH1/MW/3108; AtMyb75/Pchs_H1/MBW/5786
  9. 0 = no increase of GUS activity over the background caused by M; no enhancement caused by MB or MW over M; no enhancement caused by MBW over M or over the binary combinations.