Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus LupulusL.)

Table 2 Activation of promoters Pchs_H1 and Pchs4 with selected Myb and hop TFs in various combinations

Myb (M)	AC	M	MB*	MW**	MBW*
				Pchs_H1
HlMyb2	FN646081	(+)	+	(+)	++
s-HlMyb3	AM501509	0	+	0	++
l-HlMyb3	AM501509	0	+	0	0
HlMyb1^#	AJ876882	0	(+)	n.d.	(++)
HlMyb7	AB292244	0	0	0	0
AtMyb23	NM_123397	(+)	(+)	+	++
AtMyb12	NM_130314	++	++	++	0
AtMyb75^#	NM_104541	+	+	+	++
				Pchs4
HlMyb2	FN646081	0	0	0	(+)
s-HlMyb3	AM501509	+	+	+	0
l-HlMyb3	AM501509	+	(++)	0	0
HlMyb7	AB292244	0	0	0	0
AtMyb23	NM_123397	0	0	(+)	0
AtMyb12	NM_130314	+	0	+	0
AtMyb75^$	NM_104541	0	(+)	0	(+)

* B = HlbHLH2 (AC: FR751553)
**W = HlWD40_1 (AC: FN989721)
^$ AtPAP1;
^#not tested for Pchs4
n.d. not determined
(+) up to 10% of maximal level
+ up to 70% of maximal level
++ maximum activity observed; (++) low maximal activity; the maximal levels for M/promoter/combination/pmol GUS/mg/min: HlMyb2/PchsH1/MBW/5127; s-HlMyb3/PchsH1/MBW/1192; l-HlMyb3/Pchs4/MB/511; HlMyb1/Pchs_H1/MBW/304; AtMyb23/P_chsH1/MBW/2036; AtMyb12/P_chsH1/MW/3108; AtMyb75/Pchs_H1/MBW/5786
0 = no increase of GUS activity over the background caused by M; no enhancement caused by MB or MW over M; no enhancement caused by MBW over M or over the binary combinations.

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