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Table 1 Activation of modified variants of Pchs_H1 by hop TFs§

From: Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus LupulusL.)

Promoter variant* length (bp) Combinations M2B2W1 M3B2W1
P1chs_H1 500 100.0*** 100.0***
PMutchs_H1** 500 56.3 ± 26.0 48.0 ± 8.8
P2chs_H1 436 52.2 ± 16.7 40.7 ± 22.1
P3chs_H1 346 41.7 ± 18.8 21.1 ± 2.0
P4chs_H1 297 12.2 ± 2.3 12.2 ± 7.0
P5chs_H1 240 3.5 ± 0.6 2.1 ± 0.1
P6chs_H1 217 0.4 ± 0.1 0.3 ± 0.1
chs_H1 271 0.7 ± 0.2 0.6 ± 0.1
BGF-0# 0 << 0.1 << 0.1
P35S:BGF## 681 29.2 ± 15.6 87.5 ± 5.3
  1. § Supplemental GUS histochemical analysis together with graph containing GUS activity values is presented in additional file 8.
  2. * for details see Figure 6A; ** Myb-P box was mutated; *** 100% was 5089 ± 84 and 1721 ± 315 pmol MU/mg/min for M2B2W1 and M3B2W1, respectively
  3. # infiltration with promoterless BGF-0 vector [58] (negative control, background); ## infiltration with p35S:BGF vector (35S control - see Methods); the average activity measured for p35S:GUS without co-infiltration with MBW TFs was 1658 ± 102 pmol MU/mg/min