Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus LupulusL.)

Table 1 Activation of modified variants of Pchs_H1 by hop TFs^§

Promoter variant*	length (bp)	Combinations M2B2W1	M3B2W1
P1chs_H1	500	100.0***	100.0***
P^Mutchs_H1**	500	56.3 ± 26.0	48.0 ± 8.8
P2chs_H1	436	52.2 ± 16.7	40.7 ± 22.1
P3chs_H1	346	41.7 ± 18.8	21.1 ± 2.0
P4chs_H1	297	12.2 ± 2.3	12.2 ± 7.0
P5chs_H1	240	3.5 ± 0.6	2.1 ± 0.1
P6chs_H1	217	0.4 ± 0.1	0.3 ± 0.1
PΔchs_H1	271	0.7 ± 0.2	0.6 ± 0.1
BGF-0^#	0	<< 0.1	<< 0.1
P35S:BGF^##	681	29.2 ± 15.6	87.5 ± 5.3

§ Supplemental GUS histochemical analysis together with graph containing GUS activity values is presented in additional file 8.
* for details see Figure 6A; ** Myb-P box was mutated; *** 100% was 5089 ± 84 and 1721 ± 315 pmol MU/mg/min for M2B2W1 and M3B2W1, respectively
# infiltration with promoterless BGF-0 vector [58] (negative control, background); ## infiltration with p35S:BGF vector (35S control - see Methods); the average activity measured for p35S:GUS without co-infiltration with MBW TFs was 1658 ± 102 pmol MU/mg/min

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