Figure 5
Expression profile of 142 genes involved in the ethylene biosynthesis and signalling pathways for normal and abnormal embryos from regenerant line (CI07060) callus on embryo development medium (DEV). The relative transcript abundances were measured by real-time RT-PCR. Values are the means of the relative transcript abundance of three biological replicates. (Figure 5A) Heat map representation of the expression profile was used for values ranging as follows ≥ 1, 10-1, 10-2, 10-3and ≤ 10−4 from dark to light green. (Figure 5B) Values of relative transcript abundance in normal and abnormal embryos were analysed with XLSTAT software after log transformation. The statistical analysis was performed with an ANOVA followed by the Student Newman–Keuls test. Values with significantly high relative transcript abundances shown in red and significantly low relative transcript abundances shown in green. The non-significant genes are shown in yellow.