Nuclear and cytosolic localization of HPt2 and HPt9 in C. roseus cells. Cells were transiently transformed with HPt-GFP (HPt2 (A-D); HPt9 (I-L)) or GFP-HPt (HPt2 (E-H); HPt9 (M-P)) expressing vectors in combination with the nucleocytosolic-mcherry marker (B, F, J, N). Co-localization of the two fluorescence signals is shown in the merged image (C, G, K, O). The morphology was observed by differential interference contrast (DIC) microscopy (D, H, L, P). Scale bar = 10 μm.