Analysis of HPt9/RR13 and HPt9/RR16 interactions in C. roseus cells using BiFC assays. Cells were co-transformed using a combination of plasmids expressing HPt9/RR13 (A-H) and HPt9/RR16 (I-P) as indicated. For each combination, an additional co-transformation was performed with the CFP nuclear marker (B, F, J, N). Co-localization of the two fluorescence signals is shown in the merged image (C, G, K, O). The morphology was observed by differential interference contrast (DIC) microscopy (D, H, J, P). Scale bar = 10 μm.