Table 3 qRT PCR validation of microarray results
From: Microarray analysis of gene expression profiles in ripening pineapple fruits
Schematic Name | Microarray Result | qRT-PCR-Result | Target Gene |
|---|---|---|---|
Redox Activity and Metalloenzyme Activity | |||
contig 816 | 2.7 increase | 2.6 increase | GSPD-816 (glyceraldehyde 3-phosphate dehydrogenase, cytosolic) |
contig 3096 | 2.3 increase | 2.6 increase | ASP (peroxisome type ascorbate peroxidase) |
contig 119 | 2.7 increase | 2.3 increase | CSD2 (copper/zinc dismutase 2) |
Metalloenzyme Activity | |||
contig 180 | 1.95 increase | 3.5 increase | MET (metallothionein) |
Vitamin C Biosynthesis and Antioxidant Activity | |||
contig 3096 | 2.3 increase | 2.6 increase | ASP (ascorbate peroxidase, peroxisome type) |
contig 119 | 2.7 increase | 2.3 increase | CSD2 (copper/zinc dismutase 2) |
contig 3227 | 1.5 increase | 2.1 increase | GPP (L-galactose-1-phosphate phosphatase) |
Organic Acid Metabolism | |||
contig 816 | 2.7 increase | 2.6 increase | GSPD-816 (glyceraldehyde 3-phosphate dehydrogenase, cytosolic) |
contig 604 | 8.5 increase | 5.0 increase | MGS (monogalactosyldiacylglycerol synthase) |
Stress Related | |||
contig 35 | 6.3 decrease | 4.5 decrease | GAST (GAST-like protein) |
Cysteine-Type Peptidase and Ubiquitin Proteolysis | |||
contig 122 | 31.7 decrease | 20.6 decrease | Ac-122 (Fruit Bromelain Ac-122) |
contig 756 | 4.5 decrease | 2.0 increase | OTU (OTU-like cysteine protease family protein) |
contig 1299 | 3.1 increase | 2.1 increase | UBE2A (Ubiquitin conjugating protein) |