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Table 4 Sequence information for primers used for polymorphism and BnFLC.A10 gene expression analysis

From: A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed (Brassica napus L.)

Primer name

Sequence(5'-3')

BnFLC.A10 specific primers

Exon1-2 f

CATCCGTCGCTCTTCTTGTC

Exon1-2 r

GTTGCTTTCCATATCGATCAAG

Exon2-4 f

AACATGATGATGATCTTAAAGCCT

Exon2-4 r

CTCCAGCTGAACCAGGGAAC

Exon4-7 f

CTTGAGGAATCAAATGTCGATAA

Exon4-7 r

CGGAGATTTGTCCTGGTGAG

InDel1 f (P4 f)

GGTTCCTTTTCTTTTCGTTTGGG

InDel1 r (P4 r)

GAAGTAAAGTCGGACAAGAAGG

InDel2 f (P5 f)

CCTTCTTGTCCGACTTTACTTC

InDel2 r (P5 r)

CGTTGCTCCTACTTTGTCTATC

InDel3 (IP1IP2) f

CGTCGCTCTTCTTGTCGTCTC

InDel3 (IP1IP2) r

TATGCATCACAGCGTGTCAAA

InDel4 f

GTGTTCAGCTGTCGCTTCTAT

InDel4 r

CTAACGCTGGCTTTGATCTT

Itr1f

AATACTTCCTGCGAATCTTGTG

Itr1r

AGTTTGCTTCTAAGTCCCCAAT

SSR primers developed from JBnB75D10

25GTTA f

ACTTTCATCACCATTGCAGACA

25GTTA r

AAGAGCAGCCATTGTATCAGGT

T11 f

TTCCCAAGCTTGCTGGTACT

T11 r

GAGATTTCCCTCGCTTGATG

NIAB009 f

TACGCTAGTGAGAACACCTCCA

NIAB009 r

GCTTTAGCAAGAAAACTCGGAA

q-RT PCR primers

Prt f

TCCGTCGCTCTTCTTGTCGT

Prt r

GCTGAACCAGGGAACCCACA

actin2 F

CTGTGCCAATCTACGAGGGTTTC

actin2 R

CTTACAATTTCCCGCTCTGCTGT

18S f

GAGTATGGTCGCAAGGCTGAAA

18S r

CGCTCCACCAACTAAGAACGG