Molecular insights into how a deficiency of amylose affects carbon allocation – carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant

Table 1 Content of carbohydrates, Klason lignin and oil in BP034 and GM077

Component	Composition	BP034 (% of seed DW, n= 3*)	GM077(% of seed DW, n= 3*)
	Rhamnose**	n.d. (not detected)	n.d.
	Fucose**	n.d.	n.d.
	Arabinose**	0.45 ± 0.08	0.43 ± 0.04
	Xylose**	0.52 ± 0.04	0.49 ± 0.07
	Mannose**	0.23 ± 0.03	0.20 ± 0.04
	Galactose**	0.13 ± 0.02	0.13 ± 0.03
	Glucose**	1.03 ± 0.13	0.92 ± 0.05
	Uronic acids**	0.26 ± 0.01	0.27 ± 0.01
	Klason lignin	0.60 ± 0.50	0.56 ± 0.44
	Fructan and fructooligosaccharides	<0.10	<0.10
Total dietary fiber		3.2 ± 0.50	3.0 ± 0.32
	β-Glucan	<0.05	<0.05
	Amylose	16.8 ± 0.70 (% of seed DW)	2.6 ± 0.17 (% of seed DW)
	Amylose	24.2 (% of starch)	3.9 (% of starch)
	Amylopectin	52.5 (% of seed DW)	64.9 (% of seed DW)
Total starch		69.3 ± 0.75	67.5 ± 0.59
Oil		1.5 ± 0.21	1.5 ± 0.21
Free Glc		0.1 ± 0.00	0.2 ± 0.00
Free Suc		0.7 ± 0.15	0.9 ± 0.15

*Mean value from three independent analytic experiments using randomly selected caryopses from a pool of six plants (see table S3). **Sugar residue.

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