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Table 2 Kinetic properties of PpAOS2 with different hydroperoxy fatty acid substrates

From: Biosynthesis of allene oxides in Physcomitrella patens

Substrate

KM [μM]

Vmax [μM/min]

kcat [1/min]

kcat/KM [min-1 M-1 × 106]

9-HPOD

36 +/− 5

0.02 +/− 0.001

5

0.14

9- HPOT(n-3)

40 +/− 4

0.01 +/− 0.001

2.5

0.06

9- HPOT(n-6)

28 +/− 4.

0.03 +/− 0.002

7.5

0.27

13-HPOD

27+/− 3

0.04 +/− 0.002

10

0.37

13- HPOT(n-3)

30 +/− 5

0.02 +/− 0.002

5

0.17

13- HPOT(n-6)

42 +/− 10

0.02 +/− 0.002

5

0.12

12-HPETE

10 +/− 5

0.49 +/− 0.057

12250

1228.69

  1. Kinetic properties were determined by measuring the initial time-dependent substrate consumption at 234 nm at different substrate concentrations typically ranging from 2–100 μM. For analysis between 20 and 30 data points data points were fitted to the Michaelis-Menten equation. Note that the PpAOS2-concentration used for incubations with 12-HPETE was different (1 nM) from those used for incubations with the other substrates (100 nM). Kcat values are corrected to 100% heme occupancy from the ~4% heme content in the enzyme preparation.