Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole

Table 4 Characterization of candidate loci identified through mapping of an “enhanced sensitivity to sulfamethoxazole” (ESS) phenotype

Locus	Mutations Identified by Mapping				T-DNA Lines for Phenotypic Confirmation
Locus	Nucleotide Change^a	Strand	Genome Position^b	Amino Acid Change	Line(s) Examined	Phenotype on 0.5 μM Sulfamethoxazole^c
At2g23470	G → A	-	10000583	G219E	CS26759	Bleached
At2g24590	G → A	+	10450926	n/a^d	SALK_023090, SALK_032699C, SALK_094266	Green
At2g25320	G → A	-	10785456	G732R	SALK_072877C, SALK_007242C	Green
At2g26135	C → T	+	11130249	A43V	SALK_150146C, SALK_055187	Green
At2g27790	C → T	-	11847919	T529I	SAIL_1155_B02, SAIL_1155_E08, SALK_062215C	Green
Wildtype	n/a	n/a	n/a	n/a	n/a	Green
ESS 3-10	n/a	n/a	n/a	n/a	n/a	Bleached

^aCandidate loci were identified as described in Methods. The nucleotide change is shown in the context of the coding sequence, independent of strand orientation.
^bGenome position as defined by the Arabidopsis Information Resource.
^cSeedling phenotypes were evaluated after 14 days of growth on media containing 3 μM sulfamethoxazole. “Bleached”/”Green” describe the appearance of the majority of seedlings for each genotype and represent assessments from at least two independent experiments.
n/a = not applicable.

ISSN: 1471-2229