Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole

Table 2 Characterization of candidate loci identified through mapping of a “reduced sensitivity to sulfamethoxazole” (RSS) phenotype

Locus	Mutations Identified by Mapping				T-DNA Lines for Phenotypic Confirmation
Locus	Nucleotide Change^a	Strand	Genome Position^b	Amino Acid Change	Line(s) Examined	Phenotype on 3 μM Sulfamethoxazole^c
At5g37160	G → A	+	14705775	V90M	SALK_045992C, SALK_129697C	Bleached
At5g37830	C → T	-	15059441	S362L	SALK_078745C	Green
At5g39040	G → A	+	15629182	G529E	SALK_011884	Bleached
Wildtype	n/a	n/a	n/a	n/a	n/a	Bleached
RSS 26-1	n/a	n/a	n/a	n/a	n/a	Green

^aCandidate loci were identified as described in Methods. The nucleotide change is shown in the context of the coding sequence, independent of strand orientation.
^bGenome position as defined by the Arabidopsis Information Resource.
^cSeedling phenotypes were evaluated after 14 days of growth on media containing 3 μM sulfamethoxazole. “Bleached”/”Green” describe the appearance of the majority of seedlings for each genotype and represent assessments from at least two independent experiments.
n/a = not applicable.

ISSN: 1471-2229