Effect of pre-treatment with Pseudomonas fluorescens strain MKB158 on the accumulation of (a) abscisic acid (ABA) and (b) indole-3-acetic acid (IAA) in barley head tissues inoculated with Fusarium culmorum. Heads were treated with cells of P. fluorescens strain MKB158 or water 24 h pre-inoculation with conidia of F. culmorum strain FCF 200. Heads were harvested at various time points post-pathogen treatment, hormones were extracted and quantified by ELISA analysis. Treatments codes: C, controls treated with water and Tween20; B, bacterium plus Tween20; P, pathogen plus Tween20; B+P, bacterium plus pathogen. Results are based on two biological replicates, each containing 4 bulked technical replicates per treatment. Bars indicate standard deviation.