Table 4 Protein identification from SDS-PAGE bands in B. distachyon by MALDI-TOF-MS
LMW-GS Genes | LMW-GS subunit | Measured mass (M+H)+ | Calculated mass (M+H)+ | Missed cleavage | Peptide sequences predicted by PeptideMass | Positions |
|---|---|---|---|---|---|---|
HQ220190 | 4-1 | 845.4112 | 842.4913 | 0 | QTPEQSR | 218-224 |
| Â | Â | 1088.5128 | 1091.5411 | 0 | QCCQQLR | 211-217 |
| Â | Â | 1704.7904 | 1707.8036 | 1 | QCCQQLRQTPEQSR | 211-224 |
| Â | Â | 1765.9240 | 1765.7693 | 0 | VFLQQQCIPVAMQR | 179-192 |
| Â | Â | 1793.8608 | 1794.8394 | 0 | SQMLQQSICHVMQR | 197-210 |
| Â | Â | 2120.0925 | 2119.0759 | 1 | VFLQQQCIPVAMQRCLAR | 179-196 |
HQ220191 | 21-1 | 845.4112 | 842.5071 | 0 | QTPEQSR | 241-247 |
| Â | Â | 878.3971 | 879.4393 | 0 | QCCQQLR | 234-240 |
| Â | Â | 1704.7904 | 1707.8132 | 1 | QCCQQLRQTPEQSR | 234-247 |
| Â | Â | 1765.9240 | 1765.7466 | 0 | VFLQQQCIPVAMQR | 202-215 |
| Â | Â | 1793.8608 | 1791.7360 | 0 | SQMLQQSICHVMQR | 220-233 |
| Â | Â | 2104.0976 | 2102.0032 | 1 | VFLQQQCIPVAMQRCLAR | 202-219 |
| Â | Â | 2342.1501 | 2342.9780 | 1 | CLARSQMLQQSICHVMQR | 216-233 |
| Â | Â | 2643.3044 | 2643.3130 | 0 | MCSVNVPLYETTTSVPLGVG IGVGAY | 342-367 |
HQ220195 | 13-1 | 1479.7662 | 1482.8090 | 1 | QIPEQSRHESIR | 202-213 |
| Â | Â | 1687.9490 | 1687.8210 | 0 | AIVYSIILQQQQQR | 214-227 |
| Â | Â | 1718.8716 | 1723.8390 | 0 | VFLQQQCIPVEMQR | 163-176 |
| Â | Â | 1734.8665 | 1735.8500 | 0 | VFLQQQCIPVEMQR | 163-176 |
| Â | Â | 1823.9295 | 1828.8950 | 0 | VFLQQQCIPVEMQR | 163-176 |
| Â | Â | 2162.1031 | 2161.0060 | 1 | VFLQQQCIPVEMQRCLAR | 163-180 |
HQ220198 | 10-1 | 1593.7876 | 1595.7630 | 0 | METSCIPGLERPR | 1-13 |
| Â | Â | 1850.9404 | 1851.8900 | 0 | VFLQQQCSPIAMPQR | 109-123 |
| Â | Â | 2086.1048 | 2085.9630 | 1 | VFLQQQCSPIAMPQRLAR | 109-126 |
| Â | Â | 2102.0997 | 2102.0290 | 1 | VFLQQQCSPIAMPQRLAR | 109-126 |
| Â | Â | 3337.4388 | 3337.6920 | 0 | SQMWQQSSCHVMQQQCCQQLQQIPGQSR | 127-154 |
| Â | Â | 3960.8448 | 3963.8230 | 1 | LARSQMWQQSSCHVMQQQCCQQLQQIPGQSR | 124-154 |