Figure 1
From: miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea maysL.)
Expression analysis of ZmNAC genes and miR164 in various maize tissues, including roots, leaves, ears, leaf sheaths, stems, and male spikes, as determined by real-time PCR . The expression levels of each NAC gene in leaf was chosen as the calibrator. To perform the miR164 expression analysis, an RNA gel blot containing low-molecular-weight RNA (10 μg low molecular weight RNA per lane) was hybridized with an end-labeled DNA oligonucleotide that was complementary to miR164.