Figure 4

ChIP analysis of MdCIbHLH1 in transgenic apple callus containing either the empty vector (pBIN) or the MdCIbHLH1 gene (TGC) . (A-E) PCR products amplified from the promoters of MdCBF1 (A), MdCBF2 (B), MdCBF3 (C), MdCBF4 (C) and MdCBF5 (D) were size-fractionated by gel electrophoresis; representative gels are shown. Black lines represent the PCR products. Dilutions of input DNA were used as PCR templates to show linear amplification. (E) The ChIP product of transgenic apple callus. Input DNA was used as the control.