Figure 5

Effects of the CaMV 35S enhancer on P-INO deletion construct activity. Diagrams show the entire INO promoter and promoter deletion constructs with the first plasmid name designating the original construct, and the second name designating the same construct but with the 35S enhancer added at the extreme 5^′-end of the construct. pRJM77 is the construct with the entire P-INO [12]. The orange box represents a 60 bp minimal promoter element from the cauliflower mosaic virus (CaMV) 35S promoter. Ovule specific INO-like GUS activity indicates the number of plants exhibiting the outer integument specific GUS staining pattern shown by the full-length P-INO relative to the total number of transformants evaluated (and percentage expressing based on these values), and general 35S GUS activity indicates the number of plants exhibiting a 35S pattern of GUS activity observed in flowers relative to the total number of transformants evaluated. Results are given for constructs without (−enh) and with (+enh) the addition of the 35S enhancer. The frequencies of patterns of expression for pRJM77, pRJM209 and pRJM307 were previously described [12], and were similar to those obtained in the experimental repetitions reported here. ¹The frequencies of patterns of expression for pCGN23, pRJM199, pRJM106 and pRJM160 are those from the previous report [12]. nt, not tested.