Figure 3

Phytohormones in leaves of competing N. attenuata plants. Mean (± SE, n ≥ 4) of (A) jasmonic acid (JA) and (B) salicylic acid (SA) levels of transgenic plants grown in competition with WT plants in 2 L pots. SxN plants are crosses between irSIPK and oeNahG plants. Ten days after transfer to 2 L pots, the youngest source leaf of each plant was harvested as a control and the source-sink transition leaves were wounded with a pattern wheel (W) and treated with 20 μL 1:5 diluted Manduca sexta oral secretion (OS) and harvested 1 h after elicitation. Asteriks indicate significant differences between transgenic line and WT in one pot. (Welch two sample T-test; ***: p < 0.001; **: p < 0.01; *: p < 0.05).