Figure 1

Cloning BoFRIa and BoFRIb. (a) Southern analysis of A12 BACs identified by colony hybridisation and probed with AtFRI. Lanes 1, 4, 5, 8, 11, 12 and 13 contain clones that show homology to FRIGIDA. BACs in lanes 2, 3, 6, 7, and 10 do not cross hybridise (b) HindIII digest of six BACs probed with conserved region from exons 2 and 3 of BoFRIa. Lane 5 contains JBo72I23 from which BoFRIa was sequenced. Lane 7 contains JBo88G16. Lanes 1, 2, 3 and 6 contain four further BACs showing the same hybridization pattern as JBo88G16. Note the intensity of the hybrization is indicative of the sequence divergence between BoFRIa and BoFRIb (See 1c) (c) Comparison of the protein sequences of BoFRIa and BoFRIb with other members of the FRI sub-family. From top to bottom they are Brassica oleracea BoFRIa, Brassica oleracea BoFRIb, Thelliungiella halophila ThFRI, Arabidopsis thaliana AtFRI Arabidopsis lyrata ssp lyrata AllFRI and Arabidopsis lyrata ssp petraea AlpFRI. The N-terminal domain containing the conserved region of 37 amino acids (indicated by solid bar) that defines copies of FRIGIDA from other members of the FRI superfamily [17]. The coiled-coil domains are indicated by the red lines.