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Table 5 Mean productivity and selected phenotypic characteristics of four Golden Promise T 1 lines (two experiments) and one Scarlett line, silenced via biolistic method

From: HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes

Cultivar / line

Disturbed plants (plant number)

Number of seeds

Grain yield (g)

1000 grain weight (g)

Weight of roots (mg)

Height of plants (cm)

Spike length (cm)

Number of spikes setting seeds

Golden Promise – experiment I

5*

14 – 22 empty spikes (5/1, 5/3, 5/5)

101.3 ±29.3

2.61 ±0.56

26.2 ±2.74

90.7 ±19.0

63.3 ±3.14

8.5 ±0.57

11.5 ±7.58

6

no growth (6/4, 6/5) one spike (6/6)

112.8 ±74.6

3.68 ±2.56

32.2 ±5.65

62.6 ±16.5

58.8 ±6.83

9.13 ±0.42

6.80 ±3.70

Control GP I

 

123.5 ±47.3

4.28 ±1.24

36.85 ±7.61

52

71.28 ±2.80

8.75 ±5.02

9.03 ±4.80

Golden Promise – experiment II

4

two spikes (4/6)

192 ±85.38

5.40 ±2.72

26.6p ±5.62

51.83 ±9.64

83.33 ±3.56

8.22 ±0.66

13,2 ±9,0

5

15 - 22 empty spikes (5/9, 5/10)

172.50 ±103.78

5.85 ±3.76

33.48 ±1.77

56.00 ±9.01

76.50 ±3.02

9.22 ±0.44

13.7 ±5,5

6

no germin. (10 seeds); no growth (6/9, 6/12)

318.25 ±117.84

12.02 ±4.34

37.85 ±2.33

44.20 ±12.21

82.00 ±1.41

8.45 ±0.70

12.00 ±3.2

7

 

117.33 ±57.84

3.46p ±1.99

29.6p ±9.06

56.67 ±16.12

77.00 ±5.33

8.82 ±1.18

11.50 ±4.2

Control GP II

 

218 ±66.5

7.85 ±2.40

36.0 ±1.97

52.7 ±16.6

80.2 ±4,15

9.09 ±0.57

12.1 ±2.32

Scarlett

17

 

126.3 ±81.4

3.73 ±2.58

27.3p ±6.96

103.0p ±14.8

58.8 ±11.4

7.63 ±1.41

8.17 ±3.31

Control S (3)

 

140.0 ±24.5

5.78 ±0.96

41.4 ±0.52

58.7 ±30,0

70.0 ±3.00

8.60 ±0.70

9.33 ±3.21

  1. * – 4 to 6 plants in each line; p – P<0,05.