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Table 3 Mean productivity of T 0 plants, the weight and relative CKX activity in the roots of cv. Golden Promise transformed with a silencing cassette of HvCKX2 gene via the Agrobacterium -mediated method

From: HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes

Golden Promise (number of plants – no. of experiment)

Number of grains

Grain yield (g)

1000 grain weight (g)

Mean weight of T1 roots (mg)

Relative CKX activity in T1 roots

Agrobacterium

     

(28 – exp. 4) (4 –

148.3p ±43,9

5.25p ±1.65

35.7 ±7.75

41.3 ±11.448

0.58 ±0.37

exp. 5)

131.0 ±40.7

4.25 ±1.9

33.5 ±14.5

23.8p ±2.89

nt

Control (4 – exp.

     

4)*

90.8 ±28.6

3.15 ±1.13

34.2 ±3.97

38.3 ±8.61

0.82 ±0.23

  1. * - plants transformed with an empty (without silencing cassette) pMCG161 vector.
  2. 8 - tested for 8 lines.
  3. nt - not tested; p – P<0,05.
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BMC Plant Biology

ISSN: 1471-2229

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