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Table 1 Number of explants, selected plants and lines, and transformation efficiency after biolistic (experiment 2) and Agrobacterium -mediated transformation (experiments 4–6) with the silencing cassette/vector and an empty (pMCG161) vector as a control

From: HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes

Biolistic transformation

Cultivar

Number of experiment/cassette

Number of expl. calli plantlets rooted p. PCR+

Transformation efficiency (%)

Golden Promise

2. CKX2 line

314

189

96

6

5

1.59 (±1.24)

Scarlett

2. CKX2 line

620

414

30

3

1

0.16 (±0.59)

Agrobacterium -mediated transformation

Golden

4. pMCG/CKX2

421

186

387

36

28

6.65

Promise

4. pMCG161

100

57

63

4

3

3.00

 

5. pMCG/CKX2

75

68

106

5

4

5.33

 

6. pMCG/CKX2

440

57

4

1

1

0.23

 

Total

1036

  

45

36

3.47 (±2.82)

Scarlett

4. pMCG/CKX2

507

142

2

1

1

0.20

 

6. pMCG/CKX2

237

52

0

0

0

0

 

6. pMCG161

125

11

0

0

0

0

 

Total

869

  

1

1

0.12 (±0.12)