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Table 1 Number of explants, selected plants and lines, and transformation efficiency after biolistic (experiment 2) and Agrobacterium -mediated transformation (experiments 4–6) with the silencing cassette/vector and an empty (pMCG161) vector as a control

From: HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes

Biolistic transformation
Cultivar Number of experiment/cassette Number of expl. calli plantlets rooted p. PCR+ Transformation efficiency (%)
Golden Promise 2. CKX2 line 314 189 96 6 5 1.59 (±1.24)
Scarlett 2. CKX2 line 620 414 30 3 1 0.16 (±0.59)
Agrobacterium -mediated transformation
Golden 4. pMCG/CKX2 421 186 387 36 28 6.65
Promise 4. pMCG161 100 57 63 4 3 3.00
  5. pMCG/CKX2 75 68 106 5 4 5.33
  6. pMCG/CKX2 440 57 4 1 1 0.23
  Total 1036    45 36 3.47 (±2.82)
Scarlett 4. pMCG/CKX2 507 142 2 1 1 0.20
  6. pMCG/CKX2 237 52 0 0 0 0
  6. pMCG161 125 11 0 0 0 0
  Total 869    1 1 0.12 (±0.12)