Expression of geminiviral AC2 RNA silencing suppressor changes sugar and jasmonate responsive gene expression in transgenic tobacco plants

Table 1 Verification of microarray results using RT-qPCR

		Microarray	RT-qPCR
EST/mRNA	Description	Fold	Fold	s.e
Leaf (up-regulated transcripts
EH615198	Nicotiana tabacum, nictaba (NT1) mRNA Jasmonic acid methyl ester and ethylene-induced mRNA	27.7	22.3	4.9
EB438380	Solanum lycopersicum, Trypsin and protease inhibitor, mRNA	13.7	10.1	2.9
FG156808	Nicotiana tabacum, 1-D-deoxyxylulose 5-phosphate synthase (DXS) mRNA	3.0	2.7	0.8
Leaf (down-regulated transcript)
AY741503	Actin binding protein 1L; ABIL-1L	0.6	0.2	0.11
Leaf (non-regulated transcripts)
X67159	Nicotiana tabacum pectate lyase mRNA	0.97	0.9	0.04
Flower (up-regulated transcripts)
EB683763	Nicotiana tabacum EIG-29C mRNA	2.95	2.95	0.8
EB438380	Solanum lycopersicum Trypsin and protease inhibitor, mRNA	2.55	2.55	0.67
Flower (down-regulated transcripts)
X65700	Nicotiana tabacum osmotin, AP-24 mRNA	0.2	0.2	0.5
Flower (non-regulated transcripts)
NP917355	Nicotiana tabacum mRNA for ERF1	1.51	1.51	0.28

Some clearly up- or down-regulated genes of leaf and flower samples were tested. Statistical significance was tested using Student’s t-test (p <0.05).Fold change is indicated as a ratio of AC2/WT calculated from normalized median intensity values (n=3). Standard error of mean (s.e.) is also calculated for RT-qPCR values.

ISSN: 1471-2229