Figure 7From: Expression of geminiviral AC2 RNA silencing suppressor changes sugar and jasmonate responsive gene expression in transgenic tobacco plantsProteomic analysis of total protein samples isolated of wild type (WT) and AC2 expressing (AC2) transgenic tobacco leaves using 2D-SDS-PAGE. Proteins separated in the first dimension by isoelectric focusing were separated in another dimension by SDS-PAGE. Both wild type and AC2 expressing total protein samples were run in the second dimension in a large SDS-polyacrylamide gel. Black arrows indicate up-regulated protein spots and white arrows down-regulated protein spots. Numbers indicate previously identified protein spots by mass spectroscopic methods (1, RBCL; 2, OEE33 and 3, CYP2) [26]. Two representative 2D-SDS-PAGE results are shown.Back to article page