Proteomic analysis of total protein samples isolated of wild type (WT) and AC2 expressing (AC2) transgenic tobacco leaves using 2D-SDS-PAGE. Proteins separated in the first dimension by isoelectric focusing were separated in another dimension by SDS-PAGE. Both wild type and AC2 expressing total protein samples were run in the second dimension in a large SDS-polyacrylamide gel. Black arrows indicate up-regulated protein spots and white arrows down-regulated protein spots. Numbers indicate previously identified protein spots by mass spectroscopic methods (1, RBCL; 2, OEE33 and 3, CYP2) . Two representative 2D-SDS-PAGE results are shown.