Table 1 The list of analyzed genes and selected PCR primers used in real-time PCR.
Gene | Locus (AGI) | Forward primer (FP)Reverse primer (RP) | Tm FP or RP(°C) |
|---|---|---|---|
THI1 | AT5G54770 | CTGCTGAGGATTTGATTGTG | 49,7 |
| Â | Â | CGATTGTGTGTGGTGGTT | 48,0 |
THIC | AT2G29630 | TAGCTTACCACAAGGAGAA | 46,8 |
| Â | Â | CCAATGGAAAGAGCCACATCA | 52,4 |
TH1 | AT1G22940 | GTGATCTTCCTGACTCAT | 45,8 |
| Â | Â | CCAAAGTGCAACCAGTCC | 50,3 |
TPK | AT1G02880 | CACGATTCACTCCTCTTCTC | 51,8 |
| Â | Â | AATTCGTCGTAGATGCGATTAG | 52,0 |
KGDH | AT3G55410 | TGCGTCAAACCGGGCTTGTT | 53,8 |
| Â | Â | AGTTGGCGGGAGTTGTGGCA | 55,9 |
| Â | AT5G65750 | TGCTACCGTCGCTTTGGGCA | 55,9 |
| Â | Â | TGAAGTGACGAGGGATGACTGCG | 58,8 |
| Â | AT5G55070 | ACCGTTCTTGTCCAGGGTGTCCA | 58,8 |
| Â | Â | AGGCGGCTAGGGTGCCATCA | 57,9 |
PDH | AT5G50850 | TCGGTTTCGCCCTCAAGGCA | 55,9 |
| Â | Â | GTGGCTCGGTCAAGCGGACG | 60,0 |
| Â | AT1G59900 | TGGTGTGAGGCAGGAACGGG | 57,9 |
| Â | Â | AGAAGGCTCTGGCATTGGGCA | 56,3 |
| Â | AT3G52200 | CGCCCGGACTTCCTCGCTTC | 60,0 |
| Â | Â | GCACGCCAAATCGCAGAATGG | 56,3 |
TK | AT3G60750 | GCCGTATGAGCCGTTCCAGGT | 58,3 |
| Â | Â | GAGCCTTCTCCCAGCCAGCA | 57,9 |
| Â | AT2G45290 | ATTGGAGCCGTCACACGCCC | 58,3 |
| Â | Â | TGGTGGCATCACCCGGAGAG | 57,9 |
DXPS | AT4G15560 | TGCAAGCCATTGGACCGTGC | 55,9 |
| Â | Â | GTGCGGTTGCTGCGATGTGA | 55,9 |
EF-1 | AT1G07920 | GAGCCCAAGTTTTTGAAGA | 46,8 |
| Â | Â | CTAACAGCGAAACGTCCCA | 51,1 |