Figure 5

Impact of recombinant CCII and CCII-expressing potato lines on growth and infection capacity of Botrytis cinerea . A. Fungal growth in potato dextrose broth, in the presence or absence of purified CCII [20]. Optical density of the fungal culture at 492 nm was used as an indicator of biomass production [53]. Datapoints are the mean of three independent (biological replicate) values ± SE. B. Typical disease symptoms observed on B. cinerea-inoculated plants of line 10.4 and control line K, 12 days post-infection. C. Relative disease symptom scores after 12 days for line 9.4, line 10.4 and control line K plants inoculated with B. cinerea conidia. Data are expressed as relative cumulative symptom scores, compared to line K (100%) (see Methods).